FORMULATION AND EVALUATION OF THE EFFECTIVENESS AND STABILITY OF OINTMENT AND CREAM PREPARATIONS CONTAINING KELADI TIKUS EXTRACT (Typhonium flagelliforme) FOR TOPICAL USE
DOI:
https://doi.org/10.61677/jth.v4i2.1054Keywords:
Keladi tikus, cream, Typhonium flagelliforme, ointment, topical preparation.Abstract
Keladi tikus (Typhonium flagelliforme) is a medicinal plant with potential as an active ingredient in topical pharmaceutical preparations. This study aimed to formulate and evaluate the physical characteristics and stability of ointment and cream preparations containing keladi tikus extract. The study used an experimental method, in which keladi tikus leaves were extracted by maceration using 96% ethanol. The extract was formulated into ointment and cream preparations at concentrations of 2%, 4%, and 6%. The formulations were evaluated for organoleptic properties, homogeneity, pH, spreadability, adhesion, and physical stability during storage. The results showed that all formulations exhibited good organoleptic characteristics and homogeneous preparations. Cream spreadability ranged from 6.2–7.1 cm, while ointment spreadability ranged from 5.3–6.1 cm. Ointments demonstrated greater adhesion than creams, with values of 6.3–7.2 seconds and 4.8–5.6 seconds, respectively. During storage, all formulations maintained relatively good physical stability without significant changes in color, odor, consistency, or homogeneity. The 4% extract cream showed the most favorable characteristics, with a pH of 5.5, spreadability of 6.8 ± 0.3 cm, and adhesion of 5.2 ± 0.3 seconds. These findings indicate that keladi tikus extract has potential for development as an active ingredient in topical cream preparations.
References
Bhawar, S. B., Dighe, S. B., & Tambe, R. B. (2019). Formulation and evaluation of polyherbal ointment for wound healing and antimicrobial activity. Journal of Drug Delivery and Therapeutics, 9(3), 152–157. https://doi.org/10.22270/jddt.v9i3-s.2917
Crystalia, A. A., & Hillary, H. (2022). Anticancer activity of Typhonium flagelliforme: A systematic review. Indonesian Journal of Life Sciences, 4(1), 100–119. https://doi.org/10.54250/ijls.v4i1.99
Erdayani, E., Erlinawati, I., Nurhasanah, A. N., Hastilestari, B., & colleagues. (2023). Identification of Typhonium flagelliforme and its closely related species in Java Island using morphology and SRAP markers. AIP Conference Proceedings, 22(6), 135-143. https://doi.org/10.1063/5.0118839
Erdayani, E., Erlinawati, I., Nurhasanah, A. N., & colleagues. (2024). In silico and in vitro characterizations of rodent tuber (Typhonium flagelliforme) mutant plant isolates against FXR receptor on MCF-7 cells. Journal of Oleo Science, 73(10), 1349–1360. https://doi.org/10.5650/jos.ess24020
Harlina, E., Sandriya, A., Rostantinata, R., Nurcholis, W., Sutardi, L. N., Subangkit, M., Ridho, R., & Priosoeryanto, B. P. (2024). Antitumor activities of the Typhonium flagelliforme extract combined with natural interferons of canine and feline in mice with DMBA induced skin tumors. Jurnal Veteriner, 25(2), 301–314. https://doi.org/10.19087/jveteriner.2024.25.2.301
Khan, S., Khan, S.-U.-D., Vohra, S., Rasheed, S., Alsuwat, M. A., Hazazi, A., & Anjum, F. (2025). Deciphering the anti-cancer and anti-inflammatory activity in natural bioactive compounds of Typhonium flagelliforme: In silico approaches with special target to NEK7. Medical Oncology, 42(11), 495-507. https://doi.org/10.1007/s12032-025-03035-2
Kurnianingrum, D. N., & Zulkarnain, A. K. (2023). Optimization and physical stabilityof kembang bulan (Tithonia diversifolia [A. Gray]) extract cream formula. Jurnal Farmasi Sains dan Praktis, 9(2), 140-147. https://doi.org/10.31603/pharmacy.v9i2.8404
Lee, Y. N., Saad, N. M., & Choo, W. S. (2026). Phytochemical content, antioxidant and antibacterial properties of Typhonium flagelliforme cultivated via soil and hydroponic methods. JSFA Reports, 8(6), 76-84 https://doi.org/10.1002/jsf2.70040
Maha, H. L., Fidrianny, I., Satrialdi, & Suciati, T. (2023). An updated review of Typhonium flagelliforme: Phytochemical compound, pharmacological activities and the use of vitexin and isovitexin as flavonoid compound in cosmetics development. Pharmacia, 70(3), 673–680. https://doi.org/10.3897/pharmacia.70.e106092
Meher, R. K., Mir, S. A., Shilbayeh, S. A. R., Khan, S.-U.-D., Rasheed, S., Vohra, S., Ekka, N. J., Nayak, B., & Khan, S. (2025). Analysis of anti-cancer and anti-inflammatory activity in Typhonium flagelliforme rhizome extract by induction of apoptosis: An in-vitro study. Fitoterapia, 10(6), 182–192. https://doi.org/10.1016/j.fitote.2025.106470
Mirgane, N. A., Chandore, A., Shivankar, V., Gaikwad, Y., & Wadhawa, G. C. (2021). Phytochemical study and screening of antioxidant, anti-inflammatory Typhonium flagelliforme. Research Journal of Pharmacy and Technology, 14(5), 2686–2690. https://doi.org/10.52711/0974-360X.2021.00474
Ng, K. W., Tan, S. F., Looi, S. Y., Naimat, F., & Hamid, H. (2023). Preclinical anticancer activity of Typhonium flagelliforme (Lodd.) Blume and its potential mechanism: A systematic review. Journal of Traditional Chinese Medical Sciences, 10(4), 403–414. https://doi.org/10.1016/j.jtcms.2023.09.009
Priosoeryanto, B. P., Rostantinata, R., Harlina, E., Nurcholis, W., Sutardi, L. N., Ridho, R., & colleagues. (2020). In vitro antiproliferation activity of Typhonium flagelliforme leaves ethanol extract and its combination with canine interferons on several tumor-derived cell lines. Veterinary World, 13, 1086–1093. https://doi.org/10.14202/vetworld.2020.1086-1093
Putra, A., Riwanto, I., Putra, S. T., & Wijaya, I. (2020). Typhonium flagelliforme extract induce apoptosis in breast cancer stem cells by suppressing survivin. Journal of Cancer Research and Therapeutics, 16(6), 1302–1308. https://doi.org/10.4103/jcrt.JCRT_85_20
Sandokar, S. D., Sahare, A. Y., Bodhankar, S. S., Akare, S. C., & Wanjari, A. D. (2019). Formulation and evaluation of herbal ointment of Anogeissus latifolia bark extract. Research Journal of Pharmaceutical Dosage Forms and Technology, 11(3), 149–151. https://doi.org/10.5958/0975-4377.2019.00025.9
Sandriya, A., Priosoeryanto, B. P., Gunanti, H., Harlina, E., Rostantinata, R., Sutardi, L. N., & Ridho, R. (2021). Aktivitas antitumor ekstrak etanol daun keladi tikus (Typhonium flagelliforme) secara in vivo pada mencit. Jurnal Veteriner, 22(2), 150–158. https://doi.org/10.19087/jveteriner.2021.22.2.150
Shaikh, S. C., Sanap, D., Bhusari, D. V., Jain, S., Kochar, P. P., & Memon, F. S. (2018). Fabrication and evaluation of herbal ointment formulations of Moringa oleifera for topical delivery. Universal Journal of Pharmaceutical Research, 3(4), 92-103. https://doi.org/10.22270/ujpr.v3i4.181
Sianipar, N. F., Muflikhati, Z., & colleagues. (2025). Sequence variation and in silico protein characterization of γ-TMT gene in mutant rodent tuber (Typhonium flagelliforme Lodd). International Journal of Molecular Sciences, 26(15), 71-85 https://doi.org/10.3390/ijms26157148
Sianipar, N. F., Purnamaningsih, R., Darwati, I., & Laurent, D. (2016). Gas chromatography-mass spectrometry (GC-MS) analysis of phytochemicals of first generation gamma-irradiated Typhonium flagelliforme Lodd. mutants. Jurnal Teknologi, 7(8), 145-153. 78. https://doi.org/10.11113/jt.v78.9883
Simões, A., Veiga, F., Vitorino, C., & Figueiras, A. (2018). A tutorial for developing a topical cream formulation based on the Quality by Design approach. Journal of Pharmaceutical Sciences, 107(10), 2653–2662. https://doi.org/10.1016/j.xphs.2018.06.010
Siswanto, T., Shofiati, R., & Afifah, I. H. (2022). Typhonium flagelliforme as a cancer prevention plant—Based on in vitro, in vivo and bioinformatics research method: A systematic literature review. Nusantara Science and Technology Proceedings, 39–43. https://doi.org/10.11594/nstp.2022.2106
Usman, W. F., Widianti, R. A., & Shaleh, A. B. (2024). Formulation and physicalevaluation of ointment preparations from variations of pomegranate fruit extract (Punica granatum L.). Jurnal Farmasi & Sains Indonesia, 7(1), 240–247. https://doi.org/10.52216/jfsi.vol7no1p240-247
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